| Unlockable: | How to Unlock: |
| Astro Robin, White Cat, Death Anchor, Black Bull and Panzer Emerald | Clear Jack, Jody, Zoda, Black Shadow and Lisa Brilliant's respective stories |
| Big Fang | Clear the Novice/Silver Cup |
| Blood Hawk | Clear the Expert/Platinum Cup |
| Crazy Bear | Clear the Standard/Platinum Cup |
| Deep Claw | Clear the Novice/GoldCup |
| Elegance Liberty | Clear Rick Wheeler's Story |
| Great Star, Twin Noritta, Hyper Speeder, Queen Meteor, Little Wyvern, King Meteor | Max out Rick, Falcon, Zoda, Goroh, Lisa and Black Shadow's money (2,550,000 is the maximum) |
| Green Panther | Clear the Standard/Gold Cup |
| Iron Tiger | Clear the Standard/Bronze Cup |
| Mad Wolf | Clear S-Class in the Zero Test |
| Mighty Hurricane | Clear the Novice/Platinum Cup |
| Mighty Typhoon | Clear the Expert/Bronze Cup |
| Moon Shadow | Clear C-Class in the Zero Test |
| Night Thunder | Clear B-Class in the Zero Test |
| Red Gazelle | Clear the Expert/Gold Cup |
| Sonic Phantom | Clear A-Class in the Zero Test |
| Space Angler | Clear the Novice/Bronze Cup |
| Super Piranha | Clear the Expert/Silver Cup |
| Wild Boar | Clear the Standard/Silver Cup |
| Wonder Wasp | Clear all of Story Mode |
| Unlockable: | How to Unlock: |
| Black Shadow's Story Mode | Beat Rick Wheeler's, Captain Falcon's and Lisa Brilliant's Complete Story Modes |
| Captain Falcon's Story Mode | Beat Rick Wheeler's #2 Story Mode |
| Captain Falcon's X Stage | Beat Falcon's 4th stage after beating his Story Mode |
| Elegance Liberty | Beat Story Mode with Rick Wheeler or Captain Falcon |
| Jody Summer's Story Mode | Beat Rick Wheeler's #4 or Rick Levin's #3 Story Mode |
| Lisa Brilliant's Story Mode | Beat Samurai Goroh's #2 Story Mode |
| Platinum Cup | Beat all three cups in Novice and Standard. |
| Rich Wheeler's X Stage | Have under $1,000,000,000 after completing his 4th stage |
| Rick Levin's Story Mode | Beat Rick Wheeler's #3 Story Mode |
| Samurai Goroh's Story Mode | Beat Captain Falcon's #3 Story Mode |
| Time Attack and Training | Beat at Least One Grand Prix Course in Any Cup Circuit on Any Difficulty |
| Zero Test, A-Class | Beat All Zero Test B-Class Tests With at Least a Bronze Cup Rank |
| Zero Test, B-Class | Beat All Zero Test C-Class Tests With at Least a Bronze Cup Rank |
| Zero Test, C-Class | Beat Any Grand Prix Cup Circuit on Any Difficulty at Least Once |
| Zero Test, S-Class | Beat All Zero Test A-Class Tests With at Least a Bronze Cup Rank |
| Zoda's Story Mode | Beat Captain Falcon's #2 Story Mode |
